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. 2020 Jun 2;15(6):e0232549. doi: 10.1371/journal.pone.0232549

Table 1. Strains and plasmids used in this study.

Strains or plasmids Characteristics Reference or source
Escherichia coli
DH5α supE44 ΔlacU169(φ80 lacZΔM15) recA1 hsdR17 thi-1 relA1 [34]
S17-1(λpir) recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 [35]
Serratia marcescens
1912768R Isolated from rhizosphere soil in ginger fields This study
1912768WR Mutant from 1912768R with reduced production of prodigiosin This study
1912768W Mutant from 1912768R which is totally defective in the synthesis of prodigiosin This study
ΔrpoS Strain with the deletion of rpoS in 1912768R This study
Plasmids
pMD19-T Cloning and sequencing vector, Apr Takara
pK18mobsacB Allelic replacement vector, Knr [36]
pBBR1MCS-2 Broad-host-range cloning vector, Knr [37]