Table 1.
Primers Used for the Amplification of Genes
| Target Genes | Sequence (5/–3/) | Amplicon Size (Base Pairs) | References |
|---|---|---|---|
| bap | F:ATGCCTGAGATACAAATTAT R:GTCAATCGTAAAGGTAACG |
1449 | 21 |
| ompA | F:GTTAAAGGCGACGTAGACG R:CCAGTGTTATCTGTGTGACC |
578 | 21 |
| csuE | F:CATCTTCTATTTCGGTCCC R:CGGTCTGAGCATTGGTAA |
168 | 21 |
| blaPER-1 | F:ATGAATGTCATTATAAAAGC R:AATTTGGGCTTAGGGCAGAA |
925 | 21 |
| mcr-1 | F:AGTCCGTTTGTTCTTGTGGC R:AGATCCTTGGTCTCGGCTTG |
320 | 22 |
| mcr-2 | F:CAAGTGTGTTGGTCGCAGTT R:TCTAGCCCGACAAGCATACC |
715 | 22 |
| pmrA | F:ACTGGACATGTTGCACTCTTGT R:ATGCACTTTTATGAAGTCCCGA |
757 | 23 |
| pmrB | F:TCGGGACTTCATAAAAGTGCAT R:CAGTCACAGGTGTTCGTAATT |
722 | 23 |
| 16S_rRNA | F:CAGCTCGTGTCGTGAGATGT R:CGTAAGGGCCATGATGACTT |
150 | 23 |
| Group1ompAF306 Group1and2ompAR660 |
GATGGCGTAAATCGTGGTA CAACTTTAGCGATTTCTGG |
355 | 27 |
| Group1csuEF Group1csuER |
CTTTAGCAAACATGACCTACC TACACCCGGGTTAATCGT |
702 | 27 |
| Gp1OXA66F89 Gp1OXA66R647 |
GCGCTTCAAAATCTGATGTA GCGTATATTTTGTTTCCATTC |
559 | 27 |
| Group2ompAF378 Group1and2ompAR660 |
GACCTTTCTTATCACAACGA GGCGAACATGACCTATTT |
343 | 27 |
| Group2csuEF Group2csuER |
GGCGAACATGACCTATTT CTTCATGGCTCGTTGGTT |
580 | 27 |
| Gp2OXA69F169 Gp2OXA69R330 |
CATCAAGGTCAAACTCAA TAGCCTTTTTTCCCCATC |
162 | 27 |