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. 2020 May 28;14:2135–2147. doi: 10.2147/DDDT.S249651

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© 2020 Mao et al.

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Dioscin inhibited the proliferation, migration, and invasion of A549 and H1299 cells. (A) The cell viability of A549 and H1299 cells treated with dioscin (1, 2, 4, and 8 µM) for 24 h and 48 h was determined using a Cell Counting Kit 8 assay. The width of scratches was measured in cells treated or not with dioscin, at 0 and 24 h in A549 (B) and H1299 (C) cells. Additionally, using a Transwell migration assay and Matrigel invasion assay, the number of migrated or invaded cells treated with dioscin per chamber was counted and compared with that in the control group from three independent experiments (*p < 0.05; **p< 0.01; ***p< 0.001).