Figure 4.

Effect of dioscin on the expression of EMT markers in H1299 and A549 cells. (A) Cells were treated with dioscin (1 and 2 µM) for 24 h, and the EMT-associated biomarkers were detected using Western blotting. Typical graphs and a histogram (mean ± SD) are shown, n = 3. *p < 0.05; **p< 0.01; ***p< 0.001 compared with the control group. (B) After treatment with dioscin (1 and 2 µM) for 24 h, immunofluorescence staining was performed to determined Vimentin and E-cadherin expression in H1299 and A549 cells using confocal microscopy. Blue fluorescence indicates DAPI-labeled nuclei, and Green fluorescence indicates Vimentin or E-cadherin positive expression.