Table 1C.
Effects of various formulation parameters on the characteristics of LIN-LPN Effect of lipid composition and PEGylation (i.e. amount of DSPE-PEG)
| Soy Lecithin | DSPE-PEG | Cholesterol | PC:PE (molar) | Lipid/Polymer (weight ratio) | Size (nm)±SD | PDI±SD | Zeta potential (mV)±SD |
|---|---|---|---|---|---|---|---|
| 0.46 | 0.74 | 0.06 | 7:3 | 0.2 | 111.77±1.37 | 0.13±0.02 | −43.97±0.58 |
| 0.57 | 0.63 | 0.08 | 10:3 | 0.2 | 110±3.19 | 0.18±0.06 | −43.6±1.16 |
| 0.65 | 0.55 | 0.09 | 13:3 | 0.2 | 169.2±3.27 | 0.26±0.01 | −39.17±0.9 |
| 0.73 | 0.47 | 0.10 | 17:3 | 0.2 | 141.5±4.25 | 0.16±0.02 | −41.7±0.43 |
| 0.58 | 0.92 | 0.08 | 7:3 | 0.25 | 115.77±2.11 | 0.19±0.07 | −41.97±0.21 |
| 0.71 | 0.79 | 0.10 | 10:3 | 0.25 | 136.17±3.67 | 0.19±0.02 | −44.73±0.45 |
| 0.81 | 0.69 | 0.11 | 13:3 | 0.25 | 136.57±4.96 | 0.2±0.04 | −44.67±0.87 |
| 0.91 | 0.59 | 0.13 | 17:3 | 0.25 | 143.07±2.57 | 0.15±0.01 | −43.9±1.02 |
In each of the above samples, PLGA used was 6 mg. PC – Phosphatidylcholine (i.e. soy lecithin); PE – Phosphoethanolamine. DSPE-PEG – 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000]