Table 1.
Key resource
| Reagent or resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-GSDMD | Abcam | ab209845 |
| Rabbit monoclonal anti-pIRF3 (Ser396) | Cell signaling Technology | 29047S |
| Rabbit monoclonal anti-pIRF3 (Ser386) | Abcam | Ab76493 |
| mouse monoclonal anti-IRF3 | Santa Cruz | sc-33641 |
| Caspase-11 Antibody (17D9) | Novus | NB-120-10454 |
| Alexa Fluor® 488 Mouse anti-BrdU | BD Bioscience | 558599 |
| Mouse Anti-GAPDH antibody | Santa Cruz | Sc-47724 |
| Mouse anti-beta-actin antibody | Sigma | A5316 |
| Rabbit Anti-TBK1 | Cell signaling Tech | 3504T |
| Rabbit Anti-pTBK1 (Ser172) | Cell signaling Tech | 5483S |
| Rabbit monoclonal Anti-STING | Cell signaling Tech | 13647 |
| Rabbit monoclonal Anti-pSTING (Ser365) | Cell signaling Tech | 72971 |
| Phospho-YAP/TAZ Antibody Sampler Kit | Cell signaling Tech | 52420T |
| Rabbit monoclonal Anti-LATS1 | Cell signaling Tech | 3477S |
| Rabbit monoclonal Anti-MST 1 | Cell signaling Tech | 14946S |
| Rabbit anti-pLATS1 (Ser909) | Cell signaling Tech | 9157S |
| Rabbit monoclonal Anti-pMST1 (Thr183)/MST2 (Thr180) | Cell signaling Tech | 49332S |
| Mouse Anti-BrdU antibody | BD Biosciences | 347580 |
| Mouse Monoclonal Anti-β-Actin antibody | Sigma | A5441 |
| Rabbit Ployclonal anti-cGAS | mybiosource | MBS8291689 |
| Cyclin Antibody sample kit | Cell signaling Tech | 9869 |
| Mouse monoclonal anti-Flag | Sigma | F1804 |
| AF488 donkey anti-rat antibody | Invitrogen | A-21208 |
| AF594 donkey anti-rat antibody | Invitrogen | A-21209 |
| AF594 donkey anti-mouse antibody | Invitrogen | A-21203 |
| AF594 donkey anti-rabbit antibody | Invitrogen | A-21207 |
| AF488 donkey anti-mouse antibody | Invitrogen | A-11001 |
| Rabbit monoclonal Anti-TAZ | Cell signaling Tech | 83669S |
| Rat anti-CD31 | BD Bioscience | 550274 |
| Rat anti-CD31 | millpore sigma | CBL1337 |
| Mouse Anti-dsDNA Antibody | Millipore | MAB1293 |
| Rabbit anti-Tom20 Antibody | Santa Cruz | sc-11415 |
| Rabbit anti-IKKε antibody | Cell signaling Tech | 2905T |
| Rabit anti-pIKKε antibody (ser172) | Cell signaling Tech | 8766S |
| Mouse anti-Lamin B1 antibody | Santa Cruz | sc-56143 |
| Chemicals and Recombinant Proteins | ||
| 9-Oxo-10(9H)-acridineacetic acid (CMA) | Sigma | 17927 |
| 2′,3′-cGAMP sodium salt | Sigma | SML1229 |
| LPS | Sigma | L2630 |
| LPS | InvivoGen | tlrl-3pelps |
| BrdU | Sigma | B5002 −1G |
| Mitochondrial DNA Isolation Kit | abcam | Ab65321 |
| DNeasy Blood & Tissue Kits | QIAGEN | 69506 |
| Tetramethylrhodamine (TMRM) | ThermoFisher scientific | T668 |
| ProLong™ Gold Antifade Mountant | ThermoFisher scientific | P10144 |
| Z-VAD-FMK | Selleckchem | S7023 |
| Protease Inhibitor Cocktail | Sigma | P8340 |
| BrdU Cell Proliferation ELISA Kit | abcam | ab126556 |
| Evans Blue | Sigma | E2129 |
| iScript cDNA synthesis kit | BioRad | 1708897BUN |
| SYBR Green PCR Master Mix | ThermoFisher scientific | 4312704 |
| Phosphatase Inhibitor Cocktail | VWR | 80501-130 |
| Endothelial growth medium 2 (EBM-2) | Lonza | CC-3162 |
| Non-Radioactive Cytotoxicity Assay Kit | Promega | G1780 |
| Lipofectamine 2000 | ThermoFisher scientific | 11668019 |
| TRIZOL reagent | ThermoFisher scientific | 15596026 |
| BrdU Cell Proliferation ELISA Kit | Abcam | ab126556 |
| ECL prime immuno blotting reagent | Fisher Scientific | RPN2232 |
| RIPA Buffer | Sigma | R0278 |
| HyperSep Aminopropyl columns | ThermoFisher scientific | 60108-364 |
| Mitochondria Isolation Kit for cell | Abcam | ab110170 |
| Mitochondria Isolation Kit for Tissue | Abcam | ab110169 |
| pClneoMyc-LATS1 | Addgene | 66851 |
| pWZL Neo Myr Flag TBK1 | Addgene | #20648 |
| pcDNA4/HisMaxB-YAP1 | Addgene | #18978 |
| Flag-Gsdmd | Addgene | #80950 |
| Flag-Gsdmd-NT | Addgene | #80951 |
| Fetal Bovine Serum | Sigma | F2442 |
| Amaxa human endothelial nucleofector kit | Lonza | VPI-1001 |
| Human si-cGAS | Dharmacon | E-015607-00-0005 |
| sc-RNA negative control | Dharmacon | D-001810-10-20 |
| Human si-GSDMD | Dharmacon | E-016207-00-0005 |
| Human si-IKKε | Dharmacon | E-oo3723-00-0005 |
| LDH assay kit | Promega | G1780 |
| Tamoxifen | Sigma | T5648-1g |
| MPO assay kit | Cell Biologics | CB6937 |
| Adenosine 5′ -triphosphate disodium salt | Sigma | A2383 |
| Cell Fractionation Kit | Abcam | ab109719 |
| Kinase assay buffer | Cell signaling Tech | 9802 |
| Dynabeads Sheep Anti-Rat IgG | Invitrogen | 11035 |
| RBC Lysis buffer | Qiagen | 158904 |
| Triton-X 100 | Fisher Scientific | 151-100 |
| Mouse INF-beta duoset ELISA kit | R&D systems | DY8234-05 |
| Cells | ||
| Human lung microvascular ECs (hMVECs) | Lonza | Lonza™ CC-2527 |
| HEK293T | ATCC | Cat# CRL-3216 |