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. 2020 Apr 7;7(3):247–258. doi: 10.1093/rb/rbaa005

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© The Author(s) 2020. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Mineral formation in mineralized collagen and mineralized collagen–amnion scaffolds in normal growth media. Average mineral fill was quantified by ImageJ processing of micro-CT stacks of mineralized collagen and mineralized collagen–amnion scaffolds. (A) Mineral formation of mineralized collagen scaffolds and mineralized collagen–amnion scaffolds in normal growth media without cells at Day (−1) and with cells at Day 28. No significance was found between all samples. (B) Mineralized collagen–amnion scaffolds in normal growth media and inflammatory media. ^ indicates that the Day 28 inflammatory media group was significantly (P < 0.05) greater than the Day (−1) unseeded group. Data are expressed as mean ± standard deviation (n = 3)