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. 2020 Apr 7;7(3):247–258. doi: 10.1093/rb/rbaa005

Table 1.

Antibodies used in western blots

Protein Blocking Primary antibody Secondary antibody
β-actin (45 kDa) 5% dry milk in TBST 1:1000 in 5% dry milk (Cell Signaling Technologies, Rabbit mAB, 4967) 1:2500 in TBST, anti-rabbit IgG, HRP-linked antibody (Cell Signaling Technologies, 7074)
ERK1/2 (42–44 kDa) 1:1000 in 5% dry milk (Cell Signaling Technologies, Rabbit mAB, 9102)
p-ERK1/2 (44–42 kDa) 1:1000 in 5% dry milk (Cell Signaling Technologies, Rabbit mAB, 9101)
p38 (40 kDa) 1:1000 in 5% dry milk (Cell Signaling Technologies, Rabbit mAB, 9211)
p-p38 (43 kDa) 1:1000 in 5% dry milk (Cell Signaling Technologies, Rabbit mAB, 9215)
AKT (60 kDa) 1:1000 in 5% dry milk (Cell Signaling Technologies, Rabbit mAB, 9272)
p-AKT (60 kDa) 1:2000 in 5% dry milk (Cell Signaling Technologies, Rabbit mAB, 4060)