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. 2020 Mar 11;7(3):271–281. doi: 10.1093/rb/rbaa002

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© The Author(s) 2020. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of repeated viable cell measurement on gene expression of MC3T3-E1 cells cultured on 3D PLA scaffolds. Gene expression of BSP (A), COL I (B) and OCN (C) were analyzed by quantitative real-time PCR. Cells grown on scaffolds of the test group and the control group for 7, 14 and 21 days were used as samples. The y-axis represents fold changes of gene expression normalized by GAPDH. Each data was the mean of three samples, and three scaffolds were pooled as a sample (n = 3, total = nine scaffolds for one data)