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. 2020 Jun 3;412(24):6199–6209. doi: 10.1007/s00216-020-02702-9

Fig. 4.

Fig. 4

FAM band comparisons of pvuII-digested and undigested lateral flow strip elutions. Three different representations of the LAMP amplicon products are shown. Gel a and b are LAMP amplicons digested with pvuII (independent of LFS). Gels c, d, f, and g are eluted products from the LFS itself. Furthermore, gels c and d contain uncleaved LFS-LAMP products (pvuII−), whereas gels f and g are cleaved LFS-LAMP products (pvuII+). The FAM bands 94–114 (band C) bp and 71–80 bp (b and D) are highlighted as the HPV 16 FAM amplicons. Gel b also shows the previously sequenced bands of 146 (band A) and 92 (band B). The LFS strips that DNA was eluted from are shown in LFS images e and h of Fig. 4. The gels in panels a and b are pvuII-digested LAMP products; the lateral flow strips results are equivalent to the LFS shown in panel h