Fig. 1.

Sequence analysis, phylogeny and nature of SlWRKY23. a Alignment of the amino acid sequence of SlWRKY23 with its closest homologues in other plants. Sequences were aligned using the CLUSTAL W programme and presented using BOX shade programme. Black boxes indicate identical amino acids and grey boxes show the conserved residues. b Phylogenetic analysis of the full-length SlWRKY23 amino acid sequence with closest homologues in different plants. Analysis was carried out using the MEGA5 software with a bootstrap value of 500. c SlWRKY23 functions as a transcriptional activator. A yeast transcriptional assay was carried out using SlWRKY23 (in a fusion with GAL4DNA BD) in pGBKT7 to activate the MEL1 alpha galactosidase gene. Transformed Y₁₈₇ cells were grown on SD/Trp plate. pGBKT7 in fusion with p53 and pGADT7-T vector was used as a positive control while the pGBKT7 empty vector was used as a negative control. Colony-lift filter assay was performed to indicate positive colonies which show blue color in the presence of X-α gal