Fig. 2.

Real-time PCR analysis of SlWRKY23 transcript accumulation in different tissues and in response to different hormones. aSlWRKY23 expression in different plant tissues at different stages of development. RNA was isolated from roots and leaves of 15-day, 1-month and 2-month-old plants, flowers and seeds/pulp from red mature fruits. Reactions were run in triplicates with SlCAC as the internal control for normalization. Error bars represent ± SE of three biological replicates. Expression values were analyzed by one-way ANOVA and compared using Duncan’s multiple range test (DMRT). Values on the bar carrying different letters are significantly different. bSlWRKY23 transcript accumulation in roots of 15-day-old plants in response to different hormones. Total RNA was isolated from roots of control plants and plants treated with IAA (1 µM), BAP (1 µM), ABA (50 µM) or ACC (10 µM) at various time intervals after treatment. Reactions were run in triplicates with SlCAC as the internal control for normalization. Error bars represent ± SE of three biological replicates. Analysis was carried out as described in a