Fig. 3.

Alteration in primary root length of transgenic Arabidopsis over-expressing SlWRKY23 in response to different hormones. a Real time expression analysis of SlWRKY23 in roots of homozygous lines of transgenic Arabidopsis over-expressing SlWRKY23. UBIQUITIN10 was used as an internal control. b Effect of ACC and IAA on primary root growth. Seeds of control and transgenic SlWRKY23 lines were germinated and allowed to grow for 4 days on ½ MS medium and then transferred to medium containing either 5 µM ACC or 0.5 µM IAA and allowed to grow for another 11 days and primary root length measured. Experiments were carried in three separate sets. Data represent mean values ± SE. Significant differences are shown as *P < 0.05; **P < 0.01 and ***P < 0.001. c Effect of JA and SA on primary root growth. Seeds of control and transgenic SlWRKY23 lines were germinated and allowed to grow for 4 days on ½ MS medium and then transferred to medium containing either 10 µM JA or 10 µM SA and allowed to grow for another 5 days and primary root length measured. Experiments were carried in three separate sets. Data represent mean values ± SE. Significant differences are shown as *P < 0.05; **P < 0.01 and ***P < 0.001