Table 2.
Identification of the potential human and pig IgG-binding proteins by MALDI-TOF–MS
| Spot no.a | Identified protein | Accession no. | Theoretical pI/MWb | Experimental pI/MW | MASCOT score | Coverage (%) |
|---|---|---|---|---|---|---|
| 1 | Peptidoglycan-binding protein LysM | AKG39650.1 | 4.52/37564 | 4.2/45000 | 117 | 63 |
| 2, 3 | Enolase | AKG40756.1 | 4.66/47095 | 4.7/50000 | 143 | 36 |
| 4 | Pyruvate kinase | AKG39916.1 | 5.12/54630 | 5.2/57000 | 351 | 54 |
| 5 | Lactate dehydrogenase | AKG40380.1 | 5.05/35422 | 5.0/37000 | 258 | 42 |
| 6 | Fructose-bisphasphate aldolase | AKG39743.1 | 4.90/31155 | 4.9/32000 | 375 | 44 |
| 7 | 3-Ketoacyl-ACP reductase | AKG41028.1 | 5.53/25589 | 5.7/25000 | 263 | 29 |
aSpot numbers correspond to those indicated in Fig. 1
bTheoretical pI and MW were calculated using the Compute pI/Mw server (http://web.expasy.org/compute_pi/)