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. 2020 May 27;11:860. doi: 10.3389/fimmu.2020.00860

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Copyright © 2020 Garner-Spitzer, Poellabauer, Wagner, Guzek, Zwazl, Seidl-Friedrich, Binder, Stiasny, Kundi and Wiedermann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Memory subsets of CD4 T lymphocytes and T regulatory cells. Peripheral blood mononuclear cells were stained with fluorochrome-labeled mAbs and analyzed on a BD FACS Canto II flow cytometer. (A) Quantification of T central memory (T_CM) and (B) T effector memory (T_EM) subset before (d0) and 1 week (d7) after booster vaccination as % of total CD3⁺/CD4⁺ T cells; (C) T regulatory cells (Tregs, CD3⁺/CD4⁺/CD25⁺/FOXP3⁺) as percentage of total CD3⁺/CD4⁺ T cells before (d0) and 1 week (d7) after booster; line is arithmetic mean. Analysis of variance with linear contrasts; **p ≤ 0.01, *p ≤ 0.05.