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. 2020 May 27;11:860. doi: 10.3389/fimmu.2020.00860

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Copyright © 2020 Garner-Spitzer, Poellabauer, Wagner, Guzek, Zwazl, Seidl-Friedrich, Binder, Stiasny, Kundi and Wiedermann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantification of circulating T follicular helper cells (cTfh) and cTfh1, cTfh2, and cTfh17 subsets. Peripheral blood mononuclear cells were stained with fluorochrome-labeled mAbs and analyzed on a BD FACS Canto II flow cytometer. (A) Scatterplot of total cTfh (CD3⁺/CD4⁺/CD45RA⁻/CXCR5⁺) with respective expression of CXCR3 and/or CCR6 to distinguish cTfh1, cTfh2, and cTfh17 subsets. CXCR3⁺CCR6⁻ cell are cTfh1 cells; CXCR3⁻CCR6⁻ are cTfh2 cells, and CXCR3⁻CCR6⁺ cells are cTfh17 cells. (B) Quantification of cTfh (CD3⁺/CD4⁺/CD45RA⁻/CXCR5+) before (d0) and 1 week (d7) after booster vaccination as % of total CD4⁺ T cells. (C) cTfh1 and (D) cTfh17 cells as % of total cTfh before (d0) and 1 week (d7) after booster; line is arithmetic mean. (E) cTfh17 cells as % of total cTfh before TBE booster (d0) in females and males. Analysis of variance with linear contrasts; *p ≤ 0.05; ***p ≤ 0.05.