Figure 4.

Evaluation of anti-tumor capacity in vivo by active immunization with JT–mPD1 in a syngeneic tumor mouse model. (A) BALB/c mice either remained untreated (naïve) or were immunized as depicted. (B) Level of serum IgG antibody responses against recombinant mPD1 protein, at the time of the sacrifice of the mice immunized with the mimotope. (C) In vivo anti-tumor effect shown by bars expressing the weight of the tumors explanted upon sacrifice from all the mice in each group. The level of tumor growth reduction in the immunized mice, compared to the naïve mice, is indicated in percentages above the respective bar. Corresponding macroscopic images of representative explanted tumors are shown below each bar n = 10 for each data point. The results are representative of at least two repeated experiments. Significant differences are indicated by asterisks (*P < 0.05, ***P < 0.001).