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. 2020 May 27;11:895. doi: 10.3389/fimmu.2020.00895

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Copyright © 2020 Tobias, Battin, De Sousa Linhares, Lebens, Baier, Ambroz, Drinić, Högler, Inic-Kanada, Garner-Spitzer, Preusser, Kenner, Kundi, Zielinski, Steinberger and Wiedermann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detected levels of the pro-inflammatory cytokine tumor necrosis factor alpha (TNFα) and interleukin-6 (IL-6) in the sera of the mice actively immunized with JT–mPD1 compared to the naïve mice. The examined mice sera, prior to initiation of the immunization experiment (i.e., pre-immune bleeding), and from the sacrificed mice, were diluted 1/10, and the levels of the cytokines TNFα (A) and IL-6 (B) in the sera were examined by ELISA. The results are representative of at least two repeated experiments. ns, not significant.