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. 2020 May 27;10:816. doi: 10.3389/fonc.2020.00816

Figure 1.

Figure 1

Silencing of IL31RA suppresses BLBC progression. (A) IL31RA gene was knocked down in MDA-MB-231 and MDA-MB-157 cells by shRNA and stable clones were constructed. Expression of IL31RA and STAT3 phosphorylation was detected by western blot. (B) Cancer stem cell-like properties were determined by tumorsphere assay in vector control and IL31RA-knockdown MDA-MB-231 and MDA-MB-157 cells. Scale bar, 100 μM. Statistical analysis (mean ± SD) for numbers of tumorsphere is shown. (C) Cell migration was detected by wound healing assay in vector control and IL31RA-knockdown MDA-MB-231 cells for 48 h. Statistical data (mean ± SD) for wound closure are shown. *indicates P < 0.05, **indicates P < 0.01. (D) Cell invasion was observed by transwell assay in vector control and IL31RA-knockdown MDA-MB-231 cells. Scale bar, 100 μM. Statistical data (mean ± SD) for invaded cells are shown. ***indicates P < 0.001. Scale bar, 100 μM.