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. 2020 May 27;10:816. doi: 10.3389/fonc.2020.00816

Figure 5.

Figure 5

Twist/BRD4 induces IL31RA expression. (A) The gene expression correlation of IL31RA and Twist was analyzed based on three GEO datasets. Correlation coefficients and corresponding p-values are shown. (B) Bio-informatic analyses were performed to measure IL31RA mRNA level in breast cancer subtypes. (C) Real-time PCR experiments were conducted in a series of breast cell lines to measure the mRNA levels of Twist and IL31RA. Data presented are representative of three experiments performed as the mean ± SD. (D) Vector-T47D and Twist-T47D cells were treated with or without JQ1 (1 μM), real-time PCR and western blot analyses were performed to determine the expression of IL31RA. (E) Twist and/or BRD4 were knocked down by siRNA in MDA-MB-231 and MDA-MB-157 cells; the expression of IL31RA mRNA was measured by real-time PCR. Data presented are representative of three experiments performed as the mean ± SD. (F) BLBC cells were treated with JQ1 (1 μM), the protein levels of IL31RA and p-STAT3 were detected by western blot.