Figure 4. Reconstitution of CBC–DNA complexes for crystallization.

(A, B, C) Size exclusion profiles and SDS–PAGE analysis of HapE^P88L-mutant CBC–DNA complexes from (A, B) Aspergillus fumigatus and (C) Aspergillus nidulans. The strongest CBC target sites from A. fumigatus cccA (A) and A. nidulans cycA (B, C) promoter sequences were chosen as DNA duplexes. Size exclusion chromatography (SEC) fractions that were subjected to crystallization after a subsequent concentration step are marked in red (samples labeled 1C and 2C in the respective SDS–PAGE gels). SDS–PAGE gels were stained for protein with the GelCode Blue Stain Reagent (upper panels) before DNA staining with SYBR Gold Nucleic Acid Gel Stain (lower panels).

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