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. 2020 Apr 21;23:67–76. doi: 10.1016/j.jot.2020.03.012

Figure 2.

Figure 2

Hepcidin deficiency does not alter osteoclastic differentiation or activity. (A) Representative images of TRAP-positive cells (a marker of mature osteoclasts) differentiated from the BMMs of WT mice; (B) representative images of TRAP-positive cells (a marker of mature osteoclasts) differentiated from the BMMs of Hepcidin-KO mice; (C) statistical analysis of the number of TRAP-positive cells from the BMMs of WT and Hepcidin-KO mice. (D) Representative images of the resorption pits of mature osteoclasts from the BMMs of WT mice; (E) representative images of the resorption pits of mature osteoclasts from the BMMs of Hepcidin-KO mice; (F) statistical analysis of the resorption area of mature osteoclasts from the BMMs of WT and Hepcidin-KO mice; (G) mRNA expression of the osteoclastic markers cathepsin K; (H) mRNA expression of the osteoclastic marker Mcsfr; (I) mRNA expression of the osteoclastic markers Nfatc1; (J) mRNA expression of the osteoclastic markers TRAP during osteoclastic differentiation. Data are means ± SD from six mice. NS = not significant; WT = wild type; Hepcidin-KO = hepcidin knockout; BMM = bone marrow–derived macrophage.