Table 1.
Primer sequences used for real-time qPCR analysis.
| Gene | Forward: 5′–3′ | Reverse: 5′–3′ | Product size (bp) |
|---|---|---|---|
| Scx | CAAAGACCGTGACAGAAAGAC | CTCTCCGTGACTCTTCAGTG | 111 |
| Agg | CAAGGAAAAGGAGGTGGTACT | TCATTGGAGCGAAGGTTCT | 144 |
| Sox9 | TCTGTGGGAGCGACAACTTTA | GGAGGGAGGGAAAACAGAGAA | 104 |
| Col2a1 | AAGAGCAAGGAAAAGAAACACA | GACAGTAGACGGAGGAAAGTCA | 128 |
| Col10a1 | AATACCCTTTCTGCTGCTAATG | TGCCTTGTTCTCCTCTTACTG | 170 |
| Runx2 | GGACTGTGGTTACCGTCAT | GGAGGATTTGTGAAGACTGTT | 172 |
| GAPDH | CCCGTAGACAAAATGGTGAA | TGCCGTGAGTGGAGTCATAC | 169 |
Agg = aggrecan; Col2 = collagen type II; Col10 = collagen type X; GAPDH = glyceraldehyde 3’-phosphate dehydrogenase; Runx2 = runt-related transcription factor 2; Scx = Scleraxis; Sox9 = sex-determining region Y-box 9; qPCR = quantitative polymerase chain reaction.