Figure 1.

Liposome characteristics determine uptake capacity of moDC. (A) Schematic overview of liposomes. Liposomes contain a core of phospholipids and cholesterol, and were loaded with palmitoylated SLP (indicated in yellow), αGC (indicated in blue) or combinations of both components which resided in the lipid bilayer. (B) Representative pictures of bright-field (BF) and DiD signal in moDC over time after incubation with 100 μM SLP/αGC liposomes for 45 min at 4°C (left panel) and 60 min at 37°C (right panel). (C) Detection of MFI from DiD labeled liposomes over time in human moDC. t=0 represents incubation of moDC for 45 min at 4°C, while t = 15, t = 30 and t = 60 represent MFI after incubation at 37°C. Data is presented as mean ± SEM n = 3.