Figure 3.

Modification of liposomes with palmitoylated Le^Y enhances uptake in moDC and human skin DC. (A) Schematic overview of liposomes with Le^Y. Palmitoylated Le^Y is integrated in the lipid bilayer together with SLP and αGC. (B) Representative data of n = 3, showing binding of DC-SIGN Fc chimera and anti-Le^Y antibody to liposomes using binding ELISA. (C) Flow cytometry data on moDC loaded with DiD⁺ liposomes over time. t = 0 represents MFI after 45 min incubation at 4°C, while t = 15, t = 30 and t = 60 are measured after transfer to 37°C. Data shown as mean ± SEM n = 4, one-way ANOVA Tukey's post hoc test, ** < 0.01, *** < 0.001. (D) Percentages of DiD⁺ moDC after 45 min incubation with liposomes at 4°C (t = 0) or after transfer to 37°C (t = 15, t = 30, t = 60). Data represents mean ± SD n = 3, paired t-test, ** < 0.01. (E) Blocking effect of DC-SIGN blocking antibody AZN-D1 on liposome uptake in moDC after 60 min incubation at 37°C. Data represents mean ± SD n = 4. Statistical significance based on one-way ANOVA, Sidak's post hoc test, * < 0.05, ** < 0.01. (F) Representation of % DiD⁺ cells as a measure for uptake by different DC subsets in human skin of two donors. (G) MFI of DiD⁺ cells in different human skin subsets of two donors.