Figure 5.

Le^Y liposome-loaded DC activate primary T-cells. (A) Experimental set-up for priming of T-cells through co-culture of mature DC loaded with MART-1 containing and DC-targeted or non-targeted liposomes. (B) Representative dot plots showing MART-1_26−35L dextramer positive CD8⁺ T-cells induced by Empty, SLP-αGC, SLP-αGC-Le^Y liposomes and short MART-1_26−35L peptide 17 days after induction. (C) Percentages of MART-1_26−35L dextramer positive T-cells after co-culture with liposome-loaded and matured DC 17 days after induction. Five wells of liposome-loaded DC and primary CD8β T-cells were initiated for donor 1 (black) and four bulks for donor 2 (olive green). Data points outlined with red indicate the cultures shown as dot plot in figure 5B. Data represents mean ± SEM, one-way ANOVA Tukey's post hoc test, * < 0.05. (D) Frequency of MART-1 specific T-cells present in control conditions after co-culture with DCs loaded with short MART-1_26−35L peptide.