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. 2020 May 27;11:1012. doi: 10.3389/fimmu.2020.01012

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Copyright © 2020 Zhang, Wang, Ding, Yang, Wang, Huang, Zhu, Mellor, Hou, Zhou, Yan, Wang and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HPV16 E7 peptide in combination with polyIC and aCD40 generates more Tscm and affects the expression of EOMEs and T-bet. (A,B) Distributions of HPV16 E7 specific and non-specific CD8⁺ memory T cells in spleen and lymph node were measured by FCS analysis 1 months after prime and boost induced by immunization using various vaccine formulations. Frequencies of EOMEs (C,D) and T-bet (E,F) gated on CD8⁺ T cells in spleen and lymph node were measured by FCS analysis 1 months after prime and boost induced by immunization using various vaccine formulations. Error bars represent the mean ± SD of three independent experiments. **p < 0.01, ***p < 0.001, n.s, p > 0.05.