Figure 1.

Shikonin inhibited melanoma tumor growth in zebrafish xenograft model. Fluorescently labeled (A) A375 or (B) A2058 cells were microinjected into the yolk sac of 2 dpf zebrafish embryos. These zebrafish were then transferred randomly to 24-well plates, 10 embryos per well with 0.5 ml of embryo medium containing indicated concentrations of shikonin or sorafenib for a treatment period of 48 h. Representative images of embryos were shown as merged bright-field and fluorescent images at 0, 24, and 48 h. Relative fluorescence intensity were analyzed by Image J software. Data were shown as mean ± SD from three independent experiments, **P < 0.01. Blue arrows indicated pericardia. (C) H&E staining of A375 cell transplanted zebrafish. Tumor cells in yolk were shown (yellow squares).