Figure 4.
Sperm motility measurements of extended semen incubated with vaccine and fetal morphometrics from animals vaccinated through the intrauterine or intramuscular routes. (A) Sperm motility was evaluated in the presence of inactivated PPV, Spike and LI FliC (i.u. vaccine) and TriAdj using SCA CASA system for automatic sperm analysis and the average motility across 5 unique fields of view. (B–D) Fertilization rates and fetal morphometrics were measured 30 days after breeding following i.m. vaccination with Farrowsure B Gold vaccine (which contains PPV antigens) or i.u. vaccination with 3 vaccines each consisting of 400 μg recombinant PEDV spike protein, 200 μg recombinant LI FliC protein, and 1 × 107 BEI-inactivated PPV each formulated with 266 μg poly I:C, 533 μg HDP and 266 μg PCEP. (B) The ratio of viable fetuses divided by the CL per sow are presented. (C) The distance in mm between the crown and rump was measured for each fetus and the ratio are presented. Each data point represents the average length for the fetuses born to each gilt. (D) The average weight of the fetuses (g) from each litter are presented. Statistical analysis carried out by Kruskal-Wallis test and Dunns multiple comparisons test. Horizontal bars represent mean values.