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. 2020 May 6;24(12):6988–6999. doi: 10.1111/jcmm.15360

FIGURE 3.

FIGURE 3

Effects of silvestrol on surface markers and cytokine release of MdDCs. Human monocytes were differentiated to MdDCs for 5 d with GM‐CSF (10 ng/mL) and IL‐4 (10 ng/mL) in the presence or absence of different concentrations of silvestrol as indicated or vehicle (DMSO). A, Surface marker expression was measured by MACSQuant® Analyzer 10 in triplicates. Fold induction of the geometric mean of the fluorescence intensity was calculated by referring treated cells to vehicle controls (n = 6). B, Released cytokines in the supernatant were measured by cytometric bead array in triplicates (n = 6). For statistical analysis, one‐way ANOVA with Dunnett's multiple comparisons test (A‐B) was used. *P < .05, **P < .01, ***P < .001 indicate significant difference between silvestrol‐ and vehicle‐treated samples