TABLE 1.
Specific growth rates of best performing single-colony isolates obtained from serial transfer evolution experiments with S. cerevisiae CEN.PK113-7D on SMD and on SMD variants lacking individual B vitamins
| Strain IDa | Evolution condition | Evolution replicate | Growth rate (h−1) | % improvementb |
|---|---|---|---|---|
| IMS0721 | Complete SMD | 1 | 0.443 | 17 |
| IMS0722 | Complete SMD | 2 | 0.423 | 11 |
| IMS0723 | Complete SMD | 3 | 0.419 | 10 |
| IMS0747 | No thiamine | 1 | 0.383 | 35 |
| IMS0748 | No thiamine | 2 | 0.379 | 30 |
| IMS0749 | No thiamine | 3 | 0.379 | 38 |
| IMS0736 | No pyridoxine | 1 | 0.383 | 45 |
| IMS0737 | No pyridoxine | 2 | 0.379 | 44 |
| IMS0738 | No pyridoxine | 3 | 0.376 | 48 |
| IMS0733 | No pantothenate | 1 | 0.346 | 149 |
| IMS0734 | No pantothenate | 2 | 0.384 | 155 |
| IMS0735 | No pantothenate | 3 | 0.359 | 159 |
| IMS0724 | No nicotinic acid | 1 | 0.423 | 4 |
| IMS0725 | No nicotinic acid | 2 | 0.434 | 2 |
| IMS0726 | No nicotinic acid | 3 | 0.441 | 2 |
| IMS0730 | No inositol | 1 | 0.392 | 12 |
| IMS0731 | No inositol | 2 | 0.389 | 24 |
| IMS0732 | No inositol | 3 | 0.399 | 16 |
| IMS0727 | No pABA | 1 | 0.405 | 6 |
| IMS0728 | No pABA | 2 | 0.389 | 5 |
| IMS0729 | No pABA | 3 | 0.414 | 4 |
a
ID, identifier.
b
Percentage improvement over the specific growth rate of the parental strain after three transfers in the same medium is also shown (n = 1 for each strain).