TABLE 3.
Saccharomyces cerevisiae strains used in this study
| Strain ID | Relevant genotype or characteristic | Parental strain | Reference |
|---|---|---|---|
| CEN.PK113-7D | MATa | 17 | |
| CEN.PK113-5D | MATa ura3-52 | 17 | |
| IMX585 | MATa can1Δ::cas9-natNT2 U | CEN.PK113-7D | 73 |
| IMS0721 | MATa evolved in SMD colony 1 | CEN.PK113-7D | This study |
| IMS0722 | MATa evolved in SMD colony 2 | CEN.PK113-7D | This study |
| IMS0723 | MATa evolved in SMD colony 3 | CEN.PK113-7D | This study |
| IMS0724 | MATa evolved in Δnicotinic acidSMD colony 1 | CEN.PK113-7D | This study |
| IMS0725 | MATa evolved in Δnicotinic acidSMD colony 2 | CEN.PK113-7D | This study |
| IMS0726 | MATa evolved in Δnicotinic acidSMD colony 3 | CEN.PK113-7D | This study |
| IMS0727 | MATa evolved in ΔpabaSMD colony 1 | CEN.PK113-7D | This study |
| IMS0728 | MATa evolved in ΔpabaSMD colony 2 | CEN.PK113-7D | This study |
| IMS0729 | MATa evolved in ΔpabaSMD colony 3 | CEN.PK113-7D | This study |
| IMS0730 | MATa evolved in ΔinositolSMD colony 1 | CEN.PK113-7D | This study |
| IMS0731 | Demonstrate evolved in ΔinositolSMD colony 2 | CEN.PK113-7D | This study |
| IMS0732 | MATa evolved in ΔinositolSMD colony 3 | CEN.PK113-7D | This study |
| IMS0733 | MATa evolved in Δpantothenic acidSMD colony 1 | CEN.PK113-7D | This study |
| IMS0734 | MATa evolved in Δpantothenic acidSMD colony 2 | CEN.PK113-7D | This study |
| IMS0735 | MATa evolved in Δpantothenic acidSMD colony 3 | CEN.PK113-7D | This study |
| IMS0736 | MATa evolved in ΔpyridoxineSMD colony 1 | CEN.PK113-7D | This study |
| IMS0737 | MATa evolved in ΔpyridoxineSMD colony 2 | CEN.PK113-7D | This study |
| IMS0738 | MATa evolved in ΔpyridoxineSMD colony 3 | CEN.PK113-7D | This study |
| IMS0747 | MATa evolved in ΔthiamineSMD colony 1 | CEN.PK113-7D | This study |
| IMS0748 | MATa evolved in ΔthiamineSMD colony 2 | CEN.PK113-7D | This study |
| IMS0749 | MATa evolved in ΔthiamineSMD colony 3 | CEN.PK113-7D | This study |
| IMX1721 | MATa can1Δ::cas9-natNT2 cnb1Δ::SynPAM | IMX585 | This study |
| IMX1722 | MATa can1Δ::cas9-natNT2 pmr1Δ::SynPAM | IMX585 | This study |
| IMX1723 | MATa can1Δ::cas9-natNT2 fre2Δ::SynPAM | IMX585 | This study |
| IMX1817 | MATa can1Δ::cas9-natNT2 tup1Δ::SynPAM | IMX585 | This study |
| IMX1818 | MATa can1Δ::cas9-natNT2 isw2Δ::SynPAM | IMX585 | This study |
| IMX1819 | MATa can1Δ::cas9-natNT2 gal11Δ::SynPAM | IMX585 | This study |
| IMX1920 | MATa can1Δ::cas9-natNT2 aro7Δ::SynPAM | IMX585 | This study |
| IMX1985 | MATa can1Δ::cas9-natNT2 SynPAMΔ::CNB1L82F | IMX1721 | This study |
| IMX1986 | MATa can1Δ::cas9-natNT2 SynPAMΔ::PMR1S104Y | IMX1722 | This study |
| IMX1987 | MATa can1Δ::cas9-natNT2 SynPAMΔ::FRE2T110S | IMX1723 | This study |
| IMX1988 | MATa can1Δ::cas9-natNT2 abz1Δ::SynPAM | IMX585 | This study |
| IMX1989 | MATa can1Δ::cas9-natNT2 SynPAMΔ::ARO7L205S | IMX1920 | This study |
| IMX1990 | MATa can1Δ::cas9-natNT2 SynPAMΔ::ISW2S181stop | IMX1818 | This study |
| IMX1991 | MATa can1Δ::cas9-natNT2 SynPAMΔ::GAL11I541N | IMX1819 | This study |
| IMX1992 | MATa can1Δ::cas9-natNT2 SynPAMΔ::GAL11Q383stop | IMX1819 | This study |
| IMX2002 | MATa can1Δ::cas9-natNT2 SynPAMΔ::TUP1V374A | IMX1817 | This study |
| IMX2003 | MATa can1Δ::cas9-natNT2 SynPAMΔ::TUP1Q99stop | IMX1817 | This study |
| IMX2043 | MATa can1Δ::cas9-natNT2 SynPAMΔ::FRE2T110S pmr1Δ::PMR1S104Y | IMX1986 | This study |
| IMX2057 | MATa can1Δ::cas9-natNT2 SynPAMΔ::ABZ1R593H | IMX1988 | This study |
| IMX2066 | MATa can1Δ::cas9-natNT2 SynPAMΔ::TUP1V374A gal11Δ::SynPAM | IMX2002 | This study |
| IMX2110 | MATa can1Δ::cas9-natNT2 SynPAMΔ::GAL11Q383stop tup1Δ::SynPAM | IMX1992 | This study |
| IMX2127 | MATa can1Δ::cas9-natNT2 SynPAMΔ::GAL11Q383stop SynPAMΔ::TUP1Q99stop | IMX2110 | This study |
| IMX2128 | MATa can1Δ::cas9-natNT2 bas1Δ::hphNT1 | IMX585 | This study |
| IMX2087 | MATa can1Δ::cas9-natNT2 SynPAMΔ::TUP1V374A SynPAMΔ::GAL11I541N | IMX2066 | This study |
| IMX2135 | MATa can1Δ::cas9-natNT2 hphNT1Δ::BAS1Q152R | IMX2128 | This study |
| IMX2136 | MATa can1Δ::cas9-natNT2 hphNT1Δ::BAS1D101N | IMX2128 | This study |
| IMX2137 | MATa can1Δ::cas9-natNT2 hphNT1Δ::BAS1S41P | IMX2128 | This study |
| IMX2290 | MATa can1Δ::cas9-natNT2 YPRcTau3::pTDH3-THI4-tTDH1 | IMX585 | This study |
| IMX2291 | MATa can1Δ::cas9-natNT2 SynPAMΔ::CNB1L82F YPRcTau3::pTDH3-THI4-tTDH1 | IMX1985 | This study |
| IMX2289 | MMATa ATa can1Δ::cas9-natNT2 SPE2A278T | IMX585 | This study |
| IMX2292 | MATa can1Δ::cas9-natNT2 fms1Δ::SynPAM | IMX585 | This study |
| IMX2306 | MMATa ATa can1Δ::cas9-natNT2 SynPAMΔ::FMS1Q33K | IMX2292 | This study |
| IMX2308 | MATa can1Δ::cas9-natNT2 SynPAMΔ::GAL11Q383stop SynPAMΔ::TUP1V374A SPE2A278T | IMX2127 | This study |
| IMX2294 | MATa can1Δ::cas9-natNT2 SynPAMΔ::TUP1V374A SynPAMΔ::GAL11I541N FMS1Q33K::SynPAM | IMX2087 | This study |
| IMX2307 | MATa can1Δ::cas9-natNT2 SynPAMΔ::TUP1V374A SynPAMΔ::GAL11I541N SynPAMΔ::FMS1Q33K | IMX2294 | This study |