TABLE 2.
Primers used in this study
| Purpose | Primer | Sequence (5′–3′)a |
|---|---|---|
| Construction of accD1::pKC1139 | accD1-Fw | CCG GAATTC TCTACAACCAGGCACGGATGTC (EcoRI) |
| accD1-Rev | CCCAAGCTT GGTCGCACAGCTCGATGAAGT (HindIII) | |
| Construction and verification of DaccR | DaccR-up-Fw | CGGAATTC GCTTCTTCACGGTCATCGGGTA (EcoRI) |
| DaccR-up-Rev | GGGGTACC CAGCCTCCTTGAGGATCTGCTC (KpnI) | |
| DaccR-down-Fw | GGGGTACC CCTGCTGAACTCCACGCCACAC (KpnI) | |
| DaccR-down-Rev | CGGGATCCCGCTTCTTCTGCTCGTCGGT (BamHI) | |
| DaccR-ex-Fw | CGACATCCGTGCCTGGTTGTAG | |
| DaccR-ex-Rev | GCCACCCGTGATGAAGGTCTTG | |
| DaccR-Int-Fw | GGTCTGTACCGGCACTTC | |
| DaccR-Int-Rev | CACCACCTCCACCCACAG | |
| Complementation of DaccR mutant | DaccR-c-Fw | CGGAATTC CTCCGGTGCCTCTTGCATCCT (EcoRI) |
| DaccR-c-Rev | CGGGATCC CGCCTCGTGATCCTCGTTGAAC (BamHI) | |
| Construction of pKC-ermE-accD1A1 | EaccD1A1-Fw | GTGCCGGTTGGTAGGATCCAGCGGTGAGCATGCAAGAGGCACCGGAG |
| EaccD1A1-Rev | GACGTTGTAAAACGACGGCCAGTGCCAAGCTTGCTCATGCCGTCTCCTCCT | |
| Construction of His6-AccR | His6-AccR-Fw | CGGGATCC ATGGCCACGAGAACCGAC (BamHI) |
| His6-AccR-Rev | CGGAATTC ACAGTCGTCCAGGGCAACC (EcoRI) | |
| DNase I footprinting | accR-FAM-Fw | GTCAGCTCCGGTGCCTCTT |
| accR-Rev | TTGAGGATCTGCTCGCGG | |
| EMSAs | accR-accD1-Fw | GTCAGCTCCGGTGCCTCTT |
| accR-accD1-Rev | TTGAGGATCTGCTCGCGG | |
| accD2-Fw | CCTGGCGGTGGCTCGTCC | |
| accD2-Rev | TGCTCGGCGGTCAGTTGG | |
| accA3-SAV3336-Fw | AGACCTTGACCACATCGG | |
| accA3-SAV3336-Rev | ATGACTCCCTCTCCTTGAAAC | |
| accD3-Fw | CCGAACCTCTTCACCGAGTACC | |
| accD3-Rev | GTCAGCCATGCGTCAGTG | |
| accD4-birA-Fw | GCACCTCGGACGGCTTCAG | |
| accD4-birA-Rev | CGGCTCGGACATCGGGATG | |
| echA8-Fw | CGGCTACGGCGGCGAGTA | |
| echA8-Rev | GACGGTCGAGGCGGATGGT | |
| echA9-Fw | ATCCCGCCCGCCCGTCAG | |
| echA9-Rev | GTCCGCTCGCTCCGCCATG | |
| fadE2-chiC2-Fw | GCGGACTGAGCATCGTGTCTC | |
| fadE2-chiC2-Rev | GGCATGGCGACGCTCTCCT |
a
Underlining is used to indicate restriction enzyme sites.