TABLE 2.
Strains used in this study
| Strain | Yeast species | Genotype | Description | Reference or source |
|---|---|---|---|---|
| CBS 5679 | Schizosaccharomyces japonicus | Wild type | 22 | |
| CBS 10395 | Schizosaccharomyces pombe | Wild type | 22 | |
| W29 | Yarrowia lipolytica | Wild type | 22 | |
| CBS 1993 | Kuraishia capsulata | Wild type | 22 | |
| CBS 4732 | Ogataea polymorpha | Wild type | 22 | |
| CBS 11895 | Ogataea parapolymorpha | Wild type | 22 | |
| CBS 2499 | Brettanomyces bruxellensis | Wild type | 22 | |
| CBS 5147 | Pichia kudriavzevii | Wild type | 22 | |
| CBS 2352 | Hyphopichia burtonii | Wild type | 22 | |
| CBS 767 | Debaryomyces hansenii | Wild type | 22 | |
| CBS 6054 | Scheffersomyces stipitis | Wild type | 22 | |
| CBS 7064 | Millerozyma farinosa | Wild type | 22 | |
| CBS 111 | Wickerhamomyces ciferrii | Wild type | 22 | |
| CBS 5481 | Cyberlindnera fabianii | Wild type | 22 | |
| CBS 3082 | Lachancea kluyveri | Wild type | 22 | |
| CBS 6340 | Lachancea thermotolerans | Wild type | 22 | |
| CBS 270.75 | Eremothecium cymbalariae | Wild type | 22 | |
| CBS 6556 | Kluyveromyces marxianus | Wild type | 22 | |
| CBS 2359 | Kluyveromyces lactis | Wild type | 22 | |
| CBS 813 | Torulaspora delbrueckii | Wild type | 22 | |
| CBS 732 | Zygosaccharomyces rouxii | Wild type | 22 | |
| CBS 2163 | Vanderwaltozyma polyspora | Wild type | 22 | |
| CBS 4417 | Tetrapisispora phaffii | Wild type | 22 | |
| CBS 4309 | Naumovozyma castellii | Wild type | 22 | |
| CBS 2517 | Kazachstania africana | Wild type | 22 | |
| CBS 8638 | Kazachstania bulderi | Wild type | 22 | |
| CBS 2170 | Nakaseomyces delphensis | Wild type | 22 | |
| CBS 5792 | Saccharomyces bayanus | Wild type | 22 | |
| CBS 12357 | Saccharomyces eubayanus | Wild type | 67 | |
| CBS 10644 | Saccharomyces arboricolus | Wild type | 22 | |
| CBS 8840 | Saccharomyces kudriavzevii | Wild type | 22 | |
| CBS 8839 | Saccharomyces mikatae | Wild type | 22 | |
| CBS 1190 | Saccharomyces paradoxus | Wild type | 22 | |
| CBS 6412 | Saccharomyces cerevisiae | Wild type | Kyokai no. 7 | 22 |
| CEN.PK113-7D | Saccharomyces cerevisiae | MATa MAL2-8c SUC2 | Laboratory strain | 68 |
| S288C | Saccharomyces cerevisiae | MATα SUC2 gal2 mal mel flo1 flo8-1 hap1 ho bio1 bio6 | Laboratory strain | 69 |
| CBS 8066 | Saccharomyces cerevisiae | MATa/α HO/ho | Laboratory strain | 22 |
| Ethanol Red | Saccharomyces cerevisiae | MATa/α | Industrial bioethanol production strain | F. R. Lesaffre |
| NCYC 3233 | Saccharomyces cerevisiae | MATa/α | Brazilian bioethanol production strain PE-2 | 70 |
| IMS0481 | Saccharomyces cerevisiae | MATa evolved | Evolved CEN.PK113-7D for full biotin protrophy | 15 |
| IMX585 | Saccharomyces cerevisiae | MATa can1Δ::cas9-natNT2 | CEN.PK113-7D expressing Spycas9 | 26 |
| IMX1511 | Saccharomyces cerevisiae | MATa can1Δ::cas9-natNT2 sga1Δ::ScPYK1p-ScBIO1-ScBIO1t ScBIO1t | ScBIO1↑ | This study |
| IMX1862 | Saccharomyces cerevisiae | MATa can1Δ::cas9-natNT2 sga1Δ::ScPYK1p-YlBIO1-ScBIO1t | YlBIO1↑ | This study |
| IMX1861 | Saccharomyces cerevisiae | MATa can1Δ::cas9-natNT2 sga1Δ::ScPYK1p-PkBIO1-ScBIO1t | PkBIO1↑ | This study |
| IMX1863 | Saccharomyces cerevisiae | MATa can1Δ::cas9-natNT2 sga1Δ::ScPYK1p-WcBIO1-ScBIO1t | WcBIO1↑ | This study |
| IMX1859 | Saccharomyces cerevisiae | MATa can1Δ::cas9-natNT2 sga1Δ::ScPYK1p-CfBIO1-ScBIO1t | CfBIO1↑ | This study |
| IMX1857 | Saccharomyces cerevisiae | MATa can1Δ::cas9-natNT2 sga1Δ::ScPYK1p-TdBIO1-ScBIO1t | TdBIO1↑ | This study |
| IMX1858 | Saccharomyces cerevisiae | MATa can1Δ::cas9-natNT2 sga1Δ::ScPYK1p-LkBIO1-ScBIO1t | LkBIO1↑ | This study |
| IMX1860 | Saccharomyces cerevisiae | MATa can1Δ::cas9-natNT2 Scbio1Δ sga1Δ::ScPYK1p-CfBIO1-ScBIO1t | Scbio1Δ CfBIO1↑ | This study |
| IMK827 | Saccharomyces cerevisiae | MATa can1Δ::cas9-natNT2 Scbio1Δ | Biotin auxotroph | This study |
| IMX2212 | Saccharomyces cerevisiae | MATa can1Δ::cas9-natNT2 x-2Δ:: ScTEF1p-Venus-ScTDH1t | Venus↑ biotin auxotroph | This study |
| IMX2238 | Saccharomyces cerevisiae | MATa can1Δ::cas9-natNT2 Scbio1Δ sga1Δ::ScPYK1p-CfBIO1-ScBIO1t x-2Δ::ScPGK1p-mRuby2-ScPGK1t | mRuby2↑ biotin prototroph | This study |
| IMX2240 | Saccharomyces cerevisiae | MATa can1Δ::cas9-natNT2 Scbio1Δ x-2Δ::ScTEF1p-Venus-ScTDH1t | Venus↑ biotin auxotroph | This study |
| IMX2103 | Saccharomyces cerevisiae | sga1Δ::ScPYK1p-CfBIO1-ScBIO1t, ScPGK1p-ScBIO6-ScBIO6t | S288C CfBIO1↑ ScBIO6↑ | This study |
| IMX2104 | Saccharomyces cerevisiae | sga1Δ::ScPYK1p-CfBIO1-ScBIO1t | CBS 8066 CfBIO1↑ | This study |
| IMX2101 | Saccharomyces cerevisiae | sga1Δ::ScPYK1p-CfBIO1-ScBIO1t | Ethanol Red CfBIO1↑ | This study |
| IMX2090 | Saccharomyces cerevisiae | sga1Δ::ScPYK1p-CfBIO1-ScBIO1t | NCYC 3233 CfBIO1↑ | This study |