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. 2020 Jun 2;86(12):e00594-20. doi: 10.1128/AEM.00594-20

TABLE 2.

Sample preparation

Sample group Sampling point No. of samples Vol collected (ml)/sample Vol (ml) used for culture-based analysis/sample Campylobacter enrichment (10 ml/sample)
Processing Prescald 10 500 20 No
Postplucker 10 500 20 No
Pre-immersion chill 10 500 20 No
Post-immersion chill 10 500 20 Yes
Post-air chill 10 500 20 Yes
Shelf life Start of shelf life 15 500 20 Yes
End of shelf life 15 500 20 Yes
Environmental Scald water 4 2,000 10 Yes
Immersion chill water 4 2,000 10 Yes
Air in air chill room 1 15 2 No
Wall in air chill rooma ,b 1 100 10 Yes
Shackles in air chill rooma ,c 2 400, 600 10 Yes
a

Samples collected by using sponges. An additional 25 ml of BPW was added to each Whirl-Pak Speci-Sponge in the laboratory after sampling, yielding a mixture of 50 ml/sponge.

b

Two sponges swabbed the air chill room wall were pooled as one sample.

c

Ten sponges hung on random empty shackles moving in the air chill room, which were collected into two sealed bags. Due to time and practice in the air chill room during sampling, one bag contained four sponges, and the other contained six sponges. Consequently, this resulted in two samples collected with volumes of 400 and 600 ml, respectively.