TABLE 2.
Sample preparation
| Sample group | Sampling point | No. of samples | Vol collected (ml)/sample | Vol (ml) used for culture-based analysis/sample | Campylobacter enrichment (10 ml/sample) |
|---|---|---|---|---|---|
| Processing | Prescald | 10 | 500 | 20 | No |
| Postplucker | 10 | 500 | 20 | No | |
| Pre-immersion chill | 10 | 500 | 20 | No | |
| Post-immersion chill | 10 | 500 | 20 | Yes | |
| Post-air chill | 10 | 500 | 20 | Yes | |
| Shelf life | Start of shelf life | 15 | 500 | 20 | Yes |
| End of shelf life | 15 | 500 | 20 | Yes | |
| Environmental | Scald water | 4 | 2,000 | 10 | Yes |
| Immersion chill water | 4 | 2,000 | 10 | Yes | |
| Air in air chill room | 1 | 15 | 2 | No | |
| Wall in air chill rooma ,b | 1 | 100 | 10 | Yes | |
| Shackles in air chill rooma ,c | 2 | 400, 600 | 10 | Yes |
Samples collected by using sponges. An additional 25 ml of BPW was added to each Whirl-Pak Speci-Sponge in the laboratory after sampling, yielding a mixture of 50 ml/sponge.
Two sponges swabbed the air chill room wall were pooled as one sample.
Ten sponges hung on random empty shackles moving in the air chill room, which were collected into two sealed bags. Due to time and practice in the air chill room during sampling, one bag contained four sponges, and the other contained six sponges. Consequently, this resulted in two samples collected with volumes of 400 and 600 ml, respectively.