FIG 1.
Correlation between key characteristics of commercial pectins and the activity of each extracellular lyase. (A) Scatter plots show the specific activity of each enzyme as a function of substrate methylation, the most important substrate characteristic (n = 3). Open squares represent rhamnogalacturonan I substrates. (B) Size exclusion elution profiles are provided for a typical high-molecular-weight pectin, CP_Sigma, and the lowest-molecular-weight pectin, CP_90. (C) Standardized parameter estimates from linear models comparing the relative activity of each enzyme to the degree of methylation (DM), second-order degree of methylation (DM2), degree of acetylation (DA), number-average molecular weight (Mn), and galacturonic acid content (GalA) of each pectin. Asterisks denote significant differences (*, P < 0.05; **, P < 0.01; ***, P < 0.001).