TABLE 2.
Kinetic parameters of each extracellular lyase on three pectic substrates
| Enzyme | Parameter (unit of measurement) | Value for: |
||
|---|---|---|---|---|
| PGA-C | CP_Sigma | CP_85-C | ||
| PelA | Km (mg/ml) | 0.24 ± 0.06 | 0.43 ± 0.10 | 8.44 ± 2.58 |
| Vmax (IU/mg) | 39.7 ± 6.38 | 14.7 ± 2.42 | 32.1 ± 8.28 | |
| kcat (s−1) | (4.94 ± 0.79) × 104 | (1.83 ± 0.30) × 104 | (3.99 ± 1.03) × 104 | |
| kcat/Km (ml mg−1 s−1) | 2.04 × 105 | 4.22 × 104 | 4.73 × 103 | |
| PelB | Km (mg/ml) | 2.48 ± 0.95 | 0.55 ± 0.09 | 50.1 ± 140 |
| Vmax (IU/mg) | 458 ± 110 | 302 ± 19.6 | 745 ± 2,024 | |
| kcat (s−1) | (1.32 ± 0.32) × 106 | (8.69 ± 0.56) × 105 | (2.14 ± 5.83) × 106 | |
| kcat/Km (ml mg−1 s−1) | 5.30 × 105 | 1.57 × 106 | 4.28 × 104 | |
| PelC | Km (mg/ml) | 1.02 ± 0.23 | 0.04 ± 0.00 | 0.41 ± 0.06 |
| Vmax (IU/mg) | 15.1 ± 1.55 | 9.68 ± 0.471 | 12.8 ± 0.610 | |
| kcat (s−1) | (3.94 ± 0.41) × 104 | (2.52 ± 0.12) × 104 | (3.32 ± 0.16) × 104 | |
| kcat/Km (ml mg−1 s−1) | 3.84 × 104 | 5.49 × 105 | 8.02 × 104 | |
| PelD | Km (mg/ml) | 3.05 ± 1.95 | 0.89 ± 0.55 | 33.6 ± 159 |
| Vmax (IU/mg) | 304 ± 131 | 185 ± 81.1 | 531 ± 2,400 | |
| kcat (s−1) | (5.77 ± 2.48) × 105 | (3.51 ± 1.54) × 105 | (1.01 ± 4.55) × 106 | |
| kcat/Km (ml mg−1 s−1) | 1.89 × 105 | 3.94 × 105 | 2.99 × 104 | |
| Pnl | Km (mg/ml) | 7.61 ± 9.26 | 0.07 ± 0.00 | 0.13 ± 0.00 |
| Vmax (IU/mg) | 11.91 ± 11.91 | 59.27 ± 0.676 | 144.6 ± 1.894 | |
| kcat (s−1) | (2.52 ± 2.52) × 104 | (1.25 ± 0.01) × 105 | (3.05 ± 0.04) × 105 | |
| kcat/Km (ml mg−1 s−1) | 3.30 × 103 | 1.57 × 106 | 2.21 × 106 | |