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Primer sequences and annealing temperatures used for qPCR in this study
| Microbial target | Primers sequence (5′→3′) | Tm (°C) | Reference |
|---|---|---|---|
| Aspergillus | GTCGTCCCCTCTCCGG | 60 | 70 |
| CTGGAAAAAGATTGATTTGCG | |||
| Bacillus | TTGACATCCTCTGACAACCCT | 60 | This study |
| GAATGCTGGCAACTAAGATCA | |||
| Staphylococcus | ACGGTCTTGCTGTCACTTATA | 60 | 71 |
| TACACATATGTTCTTCCCTAATAA | |||
| Weissella | CGTGGGAAACCTACCTCTTAGCAG | 55 | 72 |
| GACCATCTCTTAGTGATAGCAGAACCAT | |||
| Zygosaccharomyces | CCTGTTTGAGCGTCATTTC | 55 | This study |
| GCACAGTCGCAAACTAAGC |
