FIG 2.

Schematic overview of the targeted proteotypic peptide in three Cry proteins present in known amounts in artificial mixtures used for the LC-MS/MS-MRM method validation. These peptides were detected in the IDA analysis and checked against in silico digestion results of the artificial protein mixture. Two proteotypic peptides were used to identify Cry1Aa and Cry6Aa, and three were used to identify Cry2Aa. The locations of the proteotypic peptides (orange bands) within full-length proteins (blue bars) are shown, with different letters corresponding to different amino acids (aa) and a number indicating the position of the amino acid in the protein. The residues in parentheses are the previous and subsequent amino acids in the protein sequence.