Figure 3.

Expression of SARS CoV‐2 spike (Sn) domains. Vero cells were transfected with pCMV3‐SP‐N‐MY Cplasmid expressing either the S1, S2, or S1 + S2 domains of S‐n tagged with the N‐MYC epitopes at their amino termini. Expression was detected with mAbs against the epitope tags at 48 hours posttransfection and compared to vehicle containing equivalent amount of lipofectamine 2000 reagent. Methanol fixed cells were incubated with mouse anti‐MYC antibody and stained with HRP staining followed by goat anti‐mouse secondary antibody incubation. Images were taken at ×10 magnification. HRP, horseradish peroxidase; SARS CoV, severe acute respiratory syndrome coronavirus; Sn, S‐new