Table 2.
Summary of milk sample results for all rRT-PCR assays for the large-scale dairy farm in Saudi Arabia.
| Variable | Farm |
|---|---|
| Duration of milk sampling (weeks) | 25 |
| Number of houses that milk samples were collected from | 19 |
| Number of pooled milk samples tested | 732 |
| Number positivea by pan-serotypic rRT-PCR assay (%) | 42 (5.7%) |
| Number positivea by A/ASIA/G-VII rRT-PCR assay (%) | 21/64b (32.8%) |
| Number positivea by O/ME-SA/Ind-2001d rRT-PCR assay (%) | 6/64b (9.4%) |
a
Positive results are those with at least one well giving a CT of ≤ 50.
b
22 samples were considered “inconclusive” (amplification was observed below the fluorescence threshold of 0.2) and were therefore also tested by the lineage-specific rRT-PCR assays.