Figure 2.

Scatter dot plots of intraplatelet vasodilator-stimulated phosphoprotein phosphorylation (P-VASP) in eight cats before and after 7 days of clopidogrel therapy. Platelet rich plasma from eight cats treated with PGE1, ADP or a combination of PGE1 and ADP was evaluated for P-VASP expression, as quantified by mean fluorescence intensity (MFI) using flow cytometry. (A) Before clopidogrel therapy, ADP-induced platelet activation in the presence of PGE₁ resulted in significant decrease in P-VASP expression compared to PGE-1 treated platelets in the absence of ADP. (B) Following clopidogrel therapy, ADP in the presence of PGE₁ did not result in significant decrease in P-VASP expression compared to PGE₁-treated platelets. ADP-induced platelet activation resulted in significant decrease in P-VASP expression regardless of clopidogrel treatment. Middle line represents the median and upper and lower lines represent the 25th and 75th quartile, respectively. *p < 0.05.