Fig. 6.

Apoptotic neutrophils increase ROS production in macrophages in an MPO-dependent manner. a ROS production in macrophages was measured through chemiluminescence upon stimulation with PMA. Untreated apoptotic neutrophils (ApoN) and those treated with ABAH (ApoN^ABAH) were added to macrophages for 24 h, followed by washing and PMA stimulation in the presence of HRP. The graph shows the mean from 3 independent experiments. b ROS production measured with the probe CM-H2DCFDA in infected macrophages stimulated with apoptotic neutrophils for 1 h. Apoptotic neutrophils were untreated or treated with the MPO inhibitor ABAH (ApoN^ABAH) and washed, before being added to the macrophages. The graph shows the mean ± SEM from 5 independent experiments. * p < 0.05 using repeated-measures ANOVA.