Table 1.
Taxonomic affiliations and production of industrial enzyme by 93 selected marine bacteria from the IMB, UMT collection as determined by 16S rDNA sequencing.
| Phylum | Bacteria ID | Closest representative bacteria | Identity (%) | Source | Amylase | Lipase | Protease | Total score | ||
|---|---|---|---|---|---|---|---|---|---|---|
| Proteobacteria Gammaproteobacteria Vibrionales |
HSC 4 | Vibrio mytili CAIM 528 | 97.91 | Horseshoe crab | 2 | 3 | 1 | 6 | ||
| HSC 7 | Vibrio neocaledonicus NC 470 | 99.85 | Horseshoe crab | 2 | 3 | 2 | 7 | |||
| HSC 8 | Vibrio neocaledonicus NC 470 | 99.70 | Horseshoe crab | 2 | 3 | 1 | 6 | |||
| HSC 10 | Vibrio neocaledonicus NC 470 | 99.85 | Horseshoe crab | 2 | 3 | 0 | 5 | |||
| HSC 13 | Vibrio alginolyticus NBRC 15,630 | 99.47 | Horseshoe crab | 2 | 3 | 1 | 6 | |||
| HSC 14 | Vibrio neocaledonicus NC 470 | 99.77 | Horseshoe crab | 2 | 3 | 1 | 6 | |||
| HSC 15 | Vibrio neocaledonicus NC 470 | 99.78 | Horseshoe crab | 2 | 3 | 2 | 7 | |||
| HSC 16 | Vibrio neocaledonicus NC 470 | 99.92 | Horseshoe crab | 2 | 3 | 2 | 7 | |||
| HSC 18 | Vibrio neocaledonicus NC 470 | 99.85 | Horseshoe crab | 2 | 3 | 1 | 6 | |||
| HSC 19 | Vibrio neocaledonicus NC 470 | 99.93 | Horseshoe crab | 2 | 3 | 1 | 6 | |||
| HSC 23 | Vibrio neocaledonicus NC 470 | 100 | Horseshoe crab | 2 | 3 | 1 | 6 | |||
| HSC 24 | Vibrio neocaledonicus NC 470 | 100 | Horseshoe crab | 2 | 3 | 0 | 5 | |||
| HSC 25 | Vibrio neocaledonicus NC 470 | 99.62 | Horseshoe crab | 2 | 3 | 1 | 6 | |||
| HSC 26 | Vibrio neocaledonicus NC 470 | 99.85 | Horseshoe crab | 2 | 3 | 0 | 5 | |||
| HSC 29 | Vibrio neocaledonicus NC 470 | 99.8 | Horseshoe crab | 2 | 3 | 1 | 6 | |||
| MNAD 3.2 | Vibrio furnissii CIP 102,972 | 99.85 | Marine water | 2 | 3 | 1 | 6 | |||
| MNAD 3.3 | Vibrio furnissii CIP 102,972 | 99.86 | Marine water | 2 | 3 | 1 | 6 | |||
| HEME 1.7.3 | Vibrio owensii LMG 25,443 | 99.92 | Jellyfish | 2 | 3 | 2 | 7 | |||
| HEME 2.4.2 | Vibrio neocaledonicus NC 470 | 99.49 | Jellyfish | 1 | 3 | 2 | 6 | |||
| HEME 2.8 | Vibrio alginolyticus NBRC 15,630 | 99.86 | Jellyfish | 1 | 3 | 2 | 6 | |||
| HEME 2.9.1 | Vibrio neocaledonicus NC 470 | 99.81 | Jellyfish | 1 | 3 | 2 | 6 | |||
| HEME 2.9.2 | Vibrio neocaledonicus NC 470 | 99.81 | Jellyfish | 1 | 3 | 2 | 6 | |||
| HEME 2.11.1 | Vibrio neocaledonicus NC 470 | 99.63 | Jellyfish | 1 | 3 | 2 | 6 | |||
| HEME 2.12.2 | Vibrio neocaledonicus NC 470 | 99.82 | Jellyfish | 1 | 3 | 2 | 6 | |||
| HEME 3.10 | Vibrio neocaledonicus NC 470 | 100 | Jellyfish | 2 | 3 | 2 | 7 | |||
| HEME 3.11 | Vibrio fluvialis NBRC 103,150 | 100 | Jellyfish | 1 | 3 | 3 | 7 | |||
| HEME 3.12 | Vibrio alginolyticus NBRC 15,630 | 99.73 | Jellyfish | 1 | 3 | 2 | 6 | |||
| MNAD 1.5.2 | Vibrio alginolyticus NBRC 15,630 | 99.43 | Jellyfish | 1 | 3 | 2 | 6 | |||
| MNAD 1.6.2 | Vibrio neocaledonicus NC 470 | 99.85 | Jellyfish | 1 | 3 | 2 | 6 | |||
| MNAD 3.7 | Vibrio alginolyticus NBRC 15,630 | 99.85 | Jellyfish | 2 | 3 | 2 | 7 | |||
| CV(M) 2.1 | Vibrio neocaledonicus NC 470 | 99.85 | Marine water | 2 | 3 | 1 | 6 | |||
| CV(M) 2.2 | Vibrio harveyi NBRC 15,634 | 100 | Marine water | 2 | 3 | 2 | 7 | |||
| CV(M) 2.3 | Vibrio neocaledonicus NC 470 | 99.77 | Marine water | 2 | 3 | 2 | 7 | |||
| CV(M) 2.5 | Vibrio neocaledonicus NC 470 | 99.93 | Marine water | 2 | 3 | 2 | 7 | |||
| CV(M) 3.3 | Vibrio neocaledonicus NC 470 | 99.40 | Marine water | 2 | 3 | 2 | 7 | |||
| CV(M) 3.6 | Vibrio alginolyticus NBRC 15,630 | 99.72 | Marine water | 2 | 3 | 1 | 6 | |||
| CV(M) 3.7 | Vibrio neocaledonicus NC 470 | 99.93 | Marine water | 2 | 3 | 2 | 7 | |||
| CV(M) 3.7.1 | Vibrio neocaledonicus NC 470 | 99.93 | Marine water | 2 | 3 | 2 | 7 | |||
| CV(H) 1.2(1) | Vibrio neocaledonicus NC 470 | 99.57 | Marine water | 2 | 3 | 0 | 5 | |||
| CV(H) 1.2(2) | Vibrio neocaledonicus NC 470 | 99.84 | Marine water | 2 | 3 | 2 | 7 | |||
| CV(H) 1.5 | Vibrio neocaledonicus NC 470 | 99.93 | Marine water | 2 | 3 | 2 | 7 | |||
| CV(H) 1.6 | Vibrio neocaledonicus NC 470 | 99.85 | Marine water | 2 | 3 | 2 | 7 | |||
| CV(H) 2.2 | Vibrio neocaledonicus NC 470 | 99.85 | Marine water | 2 | 3 | 2 | 7 | |||
| CV(H) 2.3 | Vibrio neocaledonicus NC 470 | 99.48 | Marine water | 2 | 3 | 1 | 6 | |||
| CV(H) 2.4 | Vibrio neocaledonicus NC 470 | 99.91 | Marine water | 2 | 3 | 2 | 7 | |||
| CV(H) 2.5 | Vibrio neocaledonicus NC 470 | 99.79 | Marine water | 2 | 3 | 2 | 7 | |||
| CV(H) 2.7 | Vibrio neocaledonicus NC 470 | 99.41 | Marine water | 2 | 3 | 2 | 7 | |||
| CV(H) 2.8 | Vibrio alginolyticus NBRC 15,630 | 99.71 | Marine water | 2 | 3 | 2 | 7 | |||
| CV(H) 2.9 | Vibrio neocaledonicus NC 470 | 99.49 | Marine water | 2 | 3 | 2 | 7 | |||
| CV(H) 2.10.3 | Vibrio neocaledonicus NC 470 | 99.72 | Marine water | 2 | 3 | 2 | 7 | |||
| CV(H) 2.11.2 | Vibrio neocaledonicus NC 470 | 99.85 | Marine water | 2 | 3 | 1 | 6 | |||
| CV(H) 3.1 | Vibrio neocaledonicus NC 470 | 99.39 | Marine water | 2 | 3 | 2 | 7 | |||
| CV(H) 3.2 | Vibrio neocaledonicus NC 470 | 99.86 | Marine water | 2 | 3 | 2 | 7 | |||
| CV(H) 3.5 | Vibrio alginolyticus NBRC 15,630 | 99.82 | Marine water | 2 | 3 | 2 | 7 | |||
| CV(H) 3.6 | Vibrio parahaemolyticus NBRC 12,711 | 99.50 | Marine water | 2 | 3 | 1 | 6 | |||
| CV(H) 3.7 | Vibrio alginolyticus NBRC 15,630 | 99.63 | Marine water | 2 | 3 | 2 | 7 | |||
| CV(H) 3.9 | Vibrio neocaledonicus NC 470 | 99.78 | Marine water | 2 | 3 | 2 | 7 | |||
| CV(H) 7 | Vibrio parahaemolyticus NBRC 12,711 | 99.79 | Marine water | 2 | 3 | 2 | 7 | |||
| HSC 5 | Photobacterium rosenbergii LMG 22,223 | 98.91 | Horseshoe crab | 2 | 3 | 0 | 5 | |||
| HSC 6 | Photobacterium rosenbergii LMG 22,223 | 98.22 | Horseshoe crab | 2 | 3 | 1 | 6 | |||
| Proteobacteria Gammaproteobacteria Alteromonadales |
HSC 27 | Shewanella algae JCM 21,037 | 99.49 | Horseshoe crab | 0 | 3 | 2 | 5 | ||
| HSC 30 | Shewanella algae JCM 21,037 | 98.07 | Horseshoe crab | 0 | 3 | 2 | 5 | |||
| HSC 34 | Shewanella haliotis JCM 14,758 | 99.34 | Horseshoe crab | 1 | 3 | 2 | 6 | |||
| HSC 36 | Shewanella haliotis JCM 14,758 | 100 | Horseshoe crab | 1 | 3 | 2 | 6 | |||
| HSC 37 | Shewanella haliotis JCM 14,758 | 100 | Horseshoe crab | 1 | 3 | 2 | 6 | |||
| HSC 40 | Shewanella haliotis JCM 14,758 | 100 | Horseshoe crab | 0 | 3 | 2 | 5 | |||
| HSC 41 | Shewanella haliotis JCM 14,758 | 99.93 | Horseshoe crab | 0 | 3 | 2 | 5 | |||
| HSC 42 | Shewanella haliotis JCM 14,758 | 99.85 | Horseshoe crab | 0 | 3 | 2 | 5 | |||
| HSC 51 | Shewanella haliotis JCM 14,758 | 100 | Horseshoe crab | 0 | 3 | 2 | 5 | |||
| HSC 52 | Shewanella haliotis JCM 14,758 | 100 | Horseshoe crab | 0 | 3 | 2 | 5 | |||
| Bact (M) 1.4 | Shewanella algae JCM 21,037 | 99.88 | Marine water | 0 | 3 | 2 | 5 | |||
| CV(M) 3.2.1 | Pseudoalteromonas shioyasakiensis SE 3 | 99.71 | Marine water | 0 | 3 | 2 | 5 | |||
| Proteobacteria Alphaproteobacteria |
HSC 31 | Ruegeria mobilis DSM 23,403 | 99.76 | Horseshoe crab | 2 | 3 | 2 | 7 | ||
| Firmicutes Planococcaceae |
TB 5 | Chryseomicrobium imtechense MW10 | 99.50 | Marine sediment | 2 | 3 | 3 | 8 | ||
| TB 8 | Chryseomicrobium imtechense MW10 | 99.71 | Marine sediment | 3 | 3 | 2 | 8 | |||
| TB 9 | Chryseomicrobium imtechense MW10 | 99.27 | Marine sediment | 2 | 2 | 2 | 6 | |||
| TB 11 | Chryseomicrobium palamuruense PU 1 | 98.62 | Marine sediment | 2 | 0 | 3 | 5 | |||
| TB 13 | Chryseomicrobium imtechense MW10 | 99.71 | Marine sediment | 2 | 2 | 3 | 7 | |||
| TB 24 | Chryseomicrobium imtechense MW10 | 99.79 | Marine sediment | 1 | 1 | 3 | 5 | |||
| TB 25 | Chryseomicrobium imtechense MW10 | 99.77 | Marine sediment | 2 | 3 | 0 | 5 | |||
| TB 61 | Solibacillus isronensis B3W22 | 99.20 | Mollusc | 2 | 3 | 3 | 8 | |||
| Firmicutes Bacillaceae |
TB 15 | Bacillus haikouensis C-89 | 99.75 | Marine sediment | 2 | 0 | 3 | 5 | ||
| TB 17 | Bacillus anthracis Ames | 99.85 | Marine sediment | 0 | 3 | 2 | 5 | |||
| TB 18 | Bacillus paralicheniformis KJ-16 | 99.93 | Marine sediment | 3 | 3 | 0 | 6 | |||
| TB 19 | Bacillus oryzaecorticis R1 | 100 | Marine sediment | 2 | 0 | 3 | 5 | |||
| TB 22 | Bacillus subtilis subsp. inaquosorum KCTC 13,429 | 99.92 | Marine sediment | 3 | 3 | 0 | 6 | |||
| TB 26 | Bacillus haikouensis C-89 | 99.43 | Marine sediment | 2 | 0 | 3 | 5 | |||
| TB 29 | Bacillus paralicheniformis KJ-16 | 99.93 | Marine sediment | 2 | 3 | 3 | 8 | |||
| TB 31 | Bacillus paralicheniformis KJ-16 | 99.86 | Marine sediment | 2 | 3 | 0 | 5 | |||
| TB 32 | Bacillus anthracis Ames | 98.44 | Marine sediment | 0 | 3 | 2 | 5 | |||
| TB 37 | Bacillus paralicheniformis KJ-16 | 100 | Marine sediment | 2 | 0 | 3 | 5 | |||
| Bacteriodetes | HSC 12 | Tenacibaculum mesophilum DSM 13,764 | 99.08 | Horseshoe crab | 0 | 3 | 2 | 5 | ||
| HSC 22 | Tenacibaculum litoreum CL-TF 13 | 98.13 | Horseshoe crab | 2 | 3 | 1 | 6 | |||
*The strength of enzymatic activities was represented by scoring as follows; 0, nil (no halo); 1, low (6−10 mm halo); 2, moderate (11−20 mm halo); 3, good (≥ 21 mm halo). The 16S rDNA sequences of the identified bacteria were deposited at NCBI GenBank database. The accession numbers of 16S rDNA sequences follow order from MH643590 to MH643680. The accession numbers for HSC 12 and HSC 22 are MH643682 and MH643681, respectively. The strain for all the identified bacteria isolates were catalogued based on bacteria ID.