Fig. 3.

Injury increases GSH secretion by AC.

(A and B) Changes in intracellular and extracellular GSH levels in primary AC after 6h, 16h and 24h Hx and OGD were measured in cell lysates (A) and culture media (B) respectively. Concentrations were normalized to either 1 mg total protein or 1 mL media to facilitate comparison between the different conditions and cell type. *P<0.05, **P<0.01, ***P<0.001; two-way ANOVA compared to Nx baseline. ^#P<0.05; Two-way ANOVA, compared to injury 6h baseline. Mean ± SD. n=6.