Fig. 5.

Paracrine GSH shuttling is crucial for barrier maintenance during injury. (A) AC were transfected with GCL small Interfering RNA (siGCL). A representative immunoblot after 48h transfection shows normoxic GCL expression levels. (B) The rate of GSH release by normoxic AC after GCL knockdown was measured post transfection at 24h and 48h. (C) Permeability of the EC barrier after co-culture with transfected AC under different conditions for 48h was measured by lucifer yellow flux. **P < 0.01, compared to Nx siCTRL. ^#P < 0.05, ^##P < 0.01; Student's T-test compared to injury control (siCTRL). n = 8. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)