Table 2.
Summary of Chlamydomonas Transformation with Various Constructs
| Strain | Construct | No. of APHVIII Transformantsa | No. of vfl3 Rescued Strains |
|---|---|---|---|
| vfl3-1 | VFL3 | 875 | 3 |
| VFL3-FD | 316 | 1 (partial rescued) | |
| VFL3-5E | 449 | 0 | |
| vfl3-2 | VFL3 | 666 | 8 |
| VFL3-FD | 446 | 1 (partial rescued) | |
| VFL3-5E | 970 | 0 | |
| Ex7-HA-VFL3 | 658 | 5 | |
| Ex9-HA-VFL3 | 133 | 1 | |
| Ex7-HA-VFL3-FD | 227 | 1 (partial rescued) | |
| Ex7-HA-VFL3-5E | 221 | 0 | |
| Total | 4961 | 19 |
a
Transformants are identified by co-transformation with the APHVIII gene that confers resistance to the antibiotic paromomycin.