REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
Escherichia coli adenylate cyclase deletion strain BTH101	Euromedex	N/A
E. coli MG1655 (wild type parent strain)	Storz laboratory	N/A
E. coli MG1655 ΔacrZ	Hobbs et al., 2012	GS0284
E. coli MG1655 ΔclsABC::FRT-kan-FRT (cardiolipin-deficient)	Douglas B. Weibel	N/A
E. coli MG1655 ∆acrZ::kan	Hobbs et al. 2012	GSO598
E. coli MG1655 ∆acrZ	This study	N/A
E. coli MG1655 ∆clsABC::FRT-kan-FRT (cardiolipin-deficient)	This study	N/A
E. coli MG1655 ΔclsABC::FRT-kan-FRT ΔacrZ (cardiolipin-deficient and ΔacrZ)	This study	N/A
E. coli shuffle-T7	New England Biolabs	C3029J
Chemicals, Peptides, and Recombinant Proteins
E.coli lipid extract	Avanti	100500
cardiolipin	Avanti	841199
Ortho-nitrophenyl-beta-galactoside (ONPG)	Sigma	N1127-5G
Deposited Data
AcrB in complex with AcrZ in saposin A disc and chloramphenicol	This study	PDB 6SGS EMDB 10183
AcrB in complex with AcrZ in saposin A disc + supplementary cardiolipin and chloramphenicol	This study	PDB 6SGR EMDB 10182
AcrB saposin A disc and minocycline	This study	PDB 6SGU EMDB 10185
AcrB saposin A disc + supplementary cardiolipin and minocycline	This study	PDB 6SGT EMDB 10184
AcrB with DARPin in detergent	Eicher et al., 2012	PDB 4DX5
AcrB in complex with AcrZ and puromycin in detergent	Wang et al., 2017b	PDB 5NC5
AcrB in complex with AcrZ in detergent	Du et al., 2014	PDB 4CDI
Oligonucleotides
see Table S3
Recombinant DNA
pBAD24, arabinose-inducible expression vector	Guzman et al., 1995	N/A
pBAD24-acrZ from E. coli MG1655 (GeneID: 945365) cloned into EcoRI and HindII sites	Hobbs et al., 2012	N/A
pBAD24-acrZ P16A	This study	N/A
pBAD24-acrZ P16A V17P	This study	N/A
pBAD24-acrZ P16A V18P	This study	N/A
pBAD24-acrZ P16A M19P	This study	N/A
pBAD24-acrZ P16A A20P	This study	N/A
pBAD24-acrZ M19P	This study	N/A
pBAD24-acrZ A20P	This study	N/A
pBAD24-acrZ P16G	This study	N/A
pBAD24-acrZ V15G P16G	This study	N/A
pBAD24-acrZ P16G V17G	This study	N/A
pBAD24-acrZ S7A	This study	N/A
pBAD24-acrZ V15A	This study	N/A
pBAD24-acrZ M19A	This study	N/A
pBAD24-acrZ I22A	This study	N/A
pBAD24-acrZ L23A	This study	N/A
pBAD24-acrZ I26A	This study	N/A
pBAD24-acrZ L29A	This study	N/A
pBAD24-acrZ G30A	This study	N/A
pBAD24-acrZ E31A	This study	N/A
pBAD24-acrZ F33A	This study	N/A
pEB354-acrB from Escherichia coli MG1655 (GeneID: 945108) cloned into XbaI and XhoI sites to create T25-AcrB fusion	Hobbs et al., 2012	N/A
pUT18-acrZ from Escherichia coli MG1655 (GeneID: 945365) cloned into HindIII and EcoRI sites to create AcrZ-T18 fusion	Hobbs et al., 2012	N/A
pUT18-acrZ P16A	This study	N/A
pUT18-acrZ P16A V17P	This study	N/A
pUT18-acrZ P16A V18P	This study	N/A
pUT18-acrZ P16A M19P	This study	N/A
pUT18-acrZ P16A A20P	This study	N/A
pUT18-acrZ M19P	This study	N/A
pUT18-acrZ A20P	This study	N/A
pUT18-acrZ P16G	This study	N/A
pUT18-acrZ V15G P16G	This study	N/A
pUT18-acrZ P16G V17G	This study	N/A
pUT18-acrZ S7A	This study	N/A
pUT18-acrZ V15A	This study	N/A
pUT18-acrZ M19A	This study	N/A
pUT18-acrZ I22A	This study	N/A
pUT18-acrZ L23A	This study	N/A
pUT18-acrZ I26A	This study	N/A
pUT18-acrZ L29A	This study	N/A
pUT18-acrZ G30A	This study	N/A
pUT18-acrZ E31A	This study	N/A
pUT18-acrZ F33A	This study	N/A
Software and Algorithms
GROMACS	Abraham et al., 2015	http://manual.gromacs.org/documentation/2020.1/download.html
ISOLDE	Croll, 2018	https://isolde.cimr.cam.ac.uk/
PHENIX	Liebschner et al., 2019	https://www.phenix-online.org/
RELION 3.0	Scheres, 2012, Zivanov et al., 2018	https://www3.mrc-lmb.cam.ac.uk/relion/index.php/Main_Page
Motioncor2	Zheng et al., 2017	https://emcore.ucsf.edu/ucsf-motioncor2
Gctf	Zhang, 2016	https://www.mrc-lmb.cam.ac.uk/kzhang/
Chimera	Pettersen et al., 2004	https://www.cgl.ucsf.edu/chimera/
ResMap	Kucukelbir et al., 2014	https://sourceforge.net/projects/resmap-latest/
PyMOL	DeLano, 2002	https://pymol.org/2/
Coot	Emsley et al., 2010	http://www.ccp4.ac.uk/index.php
EMRinger	Barad et al., 2015	https://github.com/fraser-lab/EMRinger
MOLPROBITY	Chen et al., 2010	http://molprobity.biochem.duke.edu
CHARMM-GUI Membrane Builder	Qi et al., 2015	http://www.charmm-gui.org/?doc=input/membrane.bilayer
LINCS	Hess et al., 1997	N/A
VMD	Humphrey et al., 1996	https://www.ks.uiuc.edu/Development/Download/download.cgi?PackageName=VMD