TABLE 2.
MIC of various compounds against Salmonella Typhimurium SL1344 ramR::aph, Escherichia coli BW25113 marR::aph, Acinetobacter baumannii AB211, and Pseudomonas aeruginosa PAO1 exposed to various compounds alone and in combination with amitriptyline
| Antibiotics | Amitriptyline concn (fraction of MIC) | MIC (μg/ml)a
|
|||
|---|---|---|---|---|---|
| SL1344 ramR::aph | BW25113 marR::aph | AB211 | PAO1 | ||
| Chloramphenicol | None | 16 | 4 | 256 | 256 |
| 1/16 | 8 | 4 | 256 | 128 | |
| 1/8 | 4 | 4 | 128 | 128 | |
| 1/4 | 2 | 2 | 64 | 128 | |
| Ciprofloxacin | None | 0.06 | 0.004 | 512 | 0.25 |
| 1/16 | 0.06 | 0.03 | 512 | 0.25 | |
| 1/8 | 0.03 | 0.008 | 512 | 0.25 | |
| 1/4 | 0.03 | 0.004 | 512 | 0.125 | |
| Nalidixic acid | None | 8 | 4 | 1,024 | 1,024 |
| 1/16 | 8 | 4 | 512 | 512 | |
| 1/8 | 4 | 4 | 512 | 256 | |
| 1/4 | 2 | 2 | 512 | 256 | |
| Tetracycline | None | 8 | 2 | 256 | 64 |
| 1/16 | 4 | 2 | 256 | 64 | |
| 1/8 | 2 | 2 | 256 | 64 | |
| 1/4 | 1 | 1 | 256 | 32 | |
| Norfloxacin | None | 0.25 | 0.03 | 512 | 1 |
| 1/16 | 0.12 | 0.06 | 512 | 1 | |
| 1/8 | 0.25 | 0.03 | 512 | 1 | |
| 1/4 | 0.25 | 0.03 | 512 | 1 | |
| Ethidium bromide | None | 512 | 128 | 256 | 2,048 |
| 1/16 | 512 | 64 | 256 | 2,048 | |
| 1/8 | 512 | 128 | 256 | 2,048 | |
| 1/4 | 256 | 64 | 128 | 1,024 | |
a
The MIC of amitriptyline was 888 μg/ml, 444 μg/ml, 222 μg/ml, and 1,775 μg/ml against SL1344 ramR::aph, BW25113 marR::aph, AB211, and PAO1, respectively. Bold font indicates a ≥2-fold decrease in MIC value in comparison to the antibiotic alone.