FIG 2.

Sequence alignment of putative Leishmania DECR and DECR-like proteins. Sequence alignment of L. major DECR (LmjF.33.0830), L. mexicana DECR (LmxM.32.0830), L. major DECR-like (LmjF.06.0930), and L. mexicana DECR-like (LmxM.06.0930) proteins with E. coli FADH (AP_003630) was performed using Geneious 10 software (MUSCLE alignment). Amino acid residues involved in substrate binding as well as cofactor coordination were identified based on a crystal structure of E. coli FADH (27) and are highlighted with colored boxes. Red boxes, residues involved in FMN coordination; blue boxes, residues of the active site responsible in substrate binding; purple boxes, residues involved in FAD coordination; green boxes, residues involved in 4 Fe-4 S cluster coordination.